Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (18): 223-230.doi: 10.11924/j.issn.1000-6850.2012-0661
Special Issue: 生物技术; 马铃薯
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Abstract:
Temperature was one of the important ecological factors for development and reproduction of Mononychellus tanajioa. In order to learn about the ecological adaptation of M. tanajoa after its invasion into our country and provide a foundation for the monitoring early warning and effective prevention as well as analyze its invasion, diffusion and ecological adaptation mechanism, this study researched the effect of extremely high-temperature (42℃) treatment on the survive and the activity changes of several protective enzymes [polyphenol oxidase (PPO), peroxidase (POD), ascorbate oxidase (AsA-POD), catalase (CAT) and superoxide dismutase (SOD)] by spectrophotometric endpoint assay after the mites in each growth period were treated for different lengths of time at 42°C. The results showed that 42℃ have important effect on the protective enzymes of M. tanajioa. Activities of PPO and CAT of larvae significantly decreased under 42℃ treatment, but that of them were increased in protonymphs and adults, especially in protonymphs. In 42℃, AsA-POD activities of larvae and adults had no changes, but that increased 3.286-fold in protonymphs and 1.923-fold in deutonymph relative to that in 25℃. High temperature of 42℃ had no effect on POD and SOD activities of M. tanajioa. These results suggested that the tolerance of M. tanajioa to high temperature could be associated with PPO, CAT and AsA-POD, but not with POD and SOD activities.
CLC Number:
S433.2
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2012-0661
https://www.casb.org.cn/EN/Y2012/V28/I18/223