Abstract: Currently, drip irrigation technology has been widely applied in wheat culture in Xinjiang. However, more than often, the positioning of drip irrigation lines and planting pattern are not uniform. Unreasonable spacing between irrigation lines not only affects planting, wastes water resources and causes difficulties in meeting the water and nutrient requirements of plants, but also has impacts on the engineering design of irrigation projects for wheat culture. Field test pit experiments were carried out in this study with 6 positioning patterns of irrigation lines for spring wheat in medium loam soils in North Xinjiang. Effects of different positioning patterns of irrigation lines in spring wheat fields on soil moisture changes and crop growth and yield were compared and analyzed. The results showed that, with the same irrigation regime, different positioning patterns of irrigation lines caused variations in the distribution characteristics of soil moisture and differences in the utilization efficiency of irrigation water by the crop. As a result, plant height, leaf area index and crop yield of spring wheat were impacted. The positioning pattern of one-pipe-two-line (A) resulted in the highest water moisture in the 0-100 cm soil layer and was more favorable for the growth and development of spring wheat, followed by the one-pipe-five-line (F) pattern. And the one-pipe-six-line (D) pattern led to the lowest soil moisture and worst plant growth. However, considering the input- output relation, the best economic benefic was achieved with the one-pipe-five-line pattern (F) followed by the one-pipe-four-line pattern (B). Taken together, the most favorable positioning pattern of drip irrigation lines for spring wheat in medium loam soil of North Xinjiang was the one-pipe-five-line pattern (F).