Abstract: For improving the flue-cured characteristic and usability of tobacco variety ‘Honghuadajinyuan’, and clarifying the effect of foliar application of potassium humate (HA-K) on the yield and quality of ‘Honghuadajinyuan’. A random block design was used in this research, HA-K was applied once in each resettling stage, fast-growing stage and budding stage; agronomic traits were evaluated in mature stage, and yield and output value were calculated after flue-cured. Results showed that: there were no significant differences between fertilizer-treated ‘Honghuadajinyuan’ and CK in yield-related leave characters; However, compared with control, the yield, output value, proportion of superior leaves, and proportion of middle and superior leaves were significantly increased by 7.25%, 9.22%, 6.23% and 8.49%, respectively; chemical components were more harmonious than that of CK, content of reducing sugar, total sugar, and chlorine were significantly decreased by 10.09%, 17.54% and 53.85%, respectively, meanwhile, total nitrogen and potassium were significantly increased by 13.58% and 21.09%, there were no significant differences in total alkaloids; the fertilizer-treated ‘Honghuadajinyuan’ maintained smoking quality of regular ‘Honghuadajinyuan’, and no significant differences in smoking score with control. In conclusion, the HA-K, as an environmental-friendly organic fertilizer, was available for improving yield and quality of flue-cured leaves in ‘Honghuadajinyuan’ production.