Abstract: Peanut is one of the four major oil crops, the main foreign exchange earning agricultural products of China, and it is also one of the agricultural commodities with strong international competitiveness. This study was carried out during 2014-2015 in the Chengdu plain area by using the high-yield new strains cultivate by Economic Crops Research Institute, Sichuan Academy of Agricultural Sciences. The field properties, yield, nutritional ingredient elements, dry matter of root and variation of soil of peanut were comparatively studied by setting 8 experiments using different gradient and mixed fertilizer, different components of fertilizer with biological manure fertilizer, farmyard manure and fertilizers. The result showed that the effect by using biological manure fertilizer with ordinary fertilizer is the best, but the application effect of using biological manure fertilizer alone or in combination with farmyard manure is relatively poor. The highest yield of peanut can be increased by 28.2%, sugar content of peanut increased by 18.9% by using biological manure fertilizer with ordinary fertilizer. The dry matter of root of peanut increased by 25.8%. The contents of N, P, K and organic matter in soil can be increased by 45.7%, 58.2%, 38.8% and 67.2% compared with the control. Biological fertilizer can improve soil, increase crop yield, enhance crop resistance, improve quality and so on. So, it has good prospects of application in peanut planting industry.