Effect of Exogenous ABA on Yield and Key Enzyme Activities of Nitrogen Metabolism of Soybean Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb16110073

Abstract

Abstract: The main purpose of this paper was to study the relationship between key enzymes of nitrogen assimilation and exogenous abscisic acid(ABA), provide theory basis for soybean production in the actual production under drought stress of soybean during seed-filling period. The drought resistance was studied in two different cultivars at seedling stage under a simulated drought stress condition by using 15 % concentration of polyethylene glycol (PEG) in vitro in order to investigate and analyze the effect of exogenous ABA under drought stress on activities of NR、GS and GOGAT activities of soybean leaf during seed-filling period. The results showed that NR and GS activity of soybean leaves were decreased sharply during the seed-filling period, GOGAT activity first decreased and then increased under the condition of normal water supply. Compared with the control, PEG treatment decreased the activity of NR、GS and GOGAT. It produced significant differences compared with CK and PEG ABA treatment in the 21 d after beginning of seed-filling to lead the lower yield of soybean. PEG ABA treatment increased the activity of NR、GS and GOGAT, they were higher than PEG treatment and they were significantly different; their activity size were increased sharply during 28 d and 35 d after beginning of seed-filling to promote the formation of soybean seed.The results indicated that exogenous hormones improved soybean tolerance under drought stress and promote the nitrogen metabolic reaction, thereby slow down the senescence of soybean leaves and provide the grain yield.

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