Biosorption of Uranium by Bacillus subtilis and Its Mechanism

doi:10.11924/j.issn.1000-6850.casb16110089

Abstract

Abstract: The thesis aims to study the enrichment capacity of uranium by Bacillus subtilis and its mechanism under culture conditions, and thus to provide a basis for the application of this bacterium in uranium pollution control. The effects of initial concentration of uranium, culture time, and inoculation amount on biosorption ability of Bacillus subtilis to uranium were discussed. The mechanism of uranium biosorption by Bacillus subtilis was discussed by FTIR, SEM, EDS, XPS as well as by studying uranium-enriched sites. The results showed that when the inoculation amount was 6%, culture time was 48 h, the biosorption could reach a balance level. When the initial concentration of UO22 was 25-100 mg/L, Bacillus subtilis could grow normally, and enriched the uranium. The cell wall was the main uranium enrichment site of Bacillus subtilis, accounting for about 89% of the total bacterial adsorption amount. SEM and EDS showed that the cell surface of Bacillus subtilis was rough, and uranium deposited on the surface and surrounding areas of Bacillus subtilis. FTIR spectra had showed the characteristic absorption peak of UO22 . And hydroxyl, amine, carboxyl and other active sites of bacterial protein took part in biosorption of Bacillus subtilis to uranium. XPS showed that U (VI) was the main form of uranium adsorbed by Bacillus subtilis. It indicated that in a certain concentration range, Bacillus subtilis had effective enrichment effect on uranium under culture conditions.

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