Abstract: The paper aims to solve the problem of dormancy of Cardiocrinum giganteum seed which is induced by the embryo afterripening. The study was carried out by means of different hormone and temperature stratification using the seed of Cardiocrinum giganteum from the Ailao Mountain Nature Reserve in Yunnan. The result showed that the addition of 50mg/L of 6-BA for soaking of 24h can promote the growth of embryo after 90d of temperature-accelerated stratification of seed of Cardiocrinum giganteum, the embryo rate was 45.873% after 120d of stratification, and the germination rate was 45.33% which was counted after 30d of germination of the first seed, and the germinating time reduced 6 days than blank control group,with the addition of 20mg/L of 6-BA, the embryo rate was 23.008% and the germination rate was 30.33% after 90d of temperature-accelerated stratification,with the addition of 13.9mg/L of SNP, the embryo rate was 27.163% and the germination rate was 31.33%.,the embryo rate of blank control group is 16.741% and the germination rate was 26.67%. In view of the embryo rate, germination rate and germinating time, the addition of 50mg/L of 6-BA after 90d of stratification for soaking of 24h would be beneficial to promoting embryo after-ripening of seed of Cardiocrinum giganteum and to hastening seed germination.