Abstract: In order to realize the hyperspectral inversion of chlorophyll content in rice leaves infected with rice blast,the chlorophyll content and hyperspectral reflectance of 85 rice leaf samples infected by rice blast were measured and analyzed. The correlation between the hyperspectral reflectance and the chlorophyll content of rice leaves infected by rice blast was also analyzed. The linear and non-linear regression techniques were then used to establish the chlorophyll content of the inversion model.The results showed that the sensitivity of the chlorophyll content to both the original spectra and the first derivative spectra occurred at 700 nm and 752 nm respectively. The regression model was based on the spectral characteristic parameter SDr had a mean square error of 1.27 and an average relative error of 10.2 %.The results showed that the spectral reflectance of rice leaves infected with rice blast was significantly different. Based on the regression model and on the spectral characteristic parameter SDr, the chlorophyll content was predicted to be high.