Abstract: The aim is to screen a group of SSR (simple sequence repeat) molecular markers with relatively high quantity and reliability which could identify the authenticity of nulli-tetrasomes lines of wheat. The single locus makers on the SSR molecular marker map of wheat were selected, and then the makers were amplified in DNA of Chinese Spring and Chinese Spring nulli-tetrasomes lines. And then the primers that produced clear and easy-identified band were screened out. The results showed that a total of 150 SSR markers on 21 linkage maps of wheat covering the long and short arms of each chromosome were selected. At last, 67 markers were selected which showed positive amplification in Chinese Spring and non- linkage nulli- tetrasomes, and negative amplification in the corresponding linkage nulli-tetrasomes. The production was the single band. This set of primers distributed evenly over all chromosomes of wheat genome, at least 3, up to 5 on each chromosome, and their bands were single and easily identified, and could be efficiently used for identifying the authenticity of Chinese Spring nulli-tetrasomes lines and other aneuploidy of wheat.