Abstract: The aim is to study the effect of different vines’cuttings and develop a new method for high-quality and high- efficiency sweet potato seedlings. The vine of short vine type‘Ganshu No.2’was used as the material, three seedling sections, including single node, two nodes and top buds with three nodes, were set up. Plug seedling production was conducted with red soil, leaf mud and pond mud. The seedling growth and seedling days and so on were detected with different seedling raising methods. The growth of leaf number, seedling length, root number and root length were also detected. The results showed that two nodes with rotten leaf soil had the fastest vine sprouting of 3 days, the highest survival rate of 86.6%, the most root number of 9.2, and the longest root length of 5.9 cm. The treatment of two nodes with rotten leaf soil had the advantages such as short sprouting time, high survival rate, low cost and so on. The seedling standard was accomplished with short time (20 days), and the propagation coefficient and seedling speed were increased. Therefore, the treatment of two nodes with rotten leaf soil is worth to be popularized.