Abstract: To solve the low rate of seed germination, the Agriophyllum squarrosum seeds from different ecological region were used to study seed germination under five treatments (GA3 treatment, H2O2 treatment,吗ethephon treatment, ultraviolet ray treatment, freezing treatment). The results showed that the five treatments had positive influence onA. squarrosum seed germination. The seed germination rate was 100% under GA3 treatment (C =2000 mg/L) and ethephon treatment (400- 600 μmol/L) in four cological regions. Under ultraviolet ray treatment, the seed germination rate increased first and then reduced with prolonging exposure to UV radiation, and reached the maximum under 24 h UV radiation. The seed germination rate was the highest under freezing treatment (- 20℃ ), the rank was Naiman Banner(96.7% ) ＞ Shapotou(95.6% ) ＞ Minqin (21.11%)＞ Dunhuang(17.78%).