Abstract: Four heavy metals, including Pb, Cd, As and Cu, in Panax notoginseng were determined by inductively coupled plasma mass spectrometry (ICP-MS) combined with wet digestion method. 27 samples of P. notoginseng were collected in 7 different regions of 3 prefectures in Yunnan Province. The bioaccumulation and transfer of heavy metals in P. notoginseng from different regions were revealed; and the associated health risk was evaluated. Green Standards of Foreign Trading Medicinal Plants & Preparations was used as the standard to evaluate the pollution status of Pb, Cd, As and Cu in P. notoginseng. The results showed that the content of Pb, Cd, As and Cu exceeded the limit of the standard by respectively 0%, 62.96%, 37.04% and 0% in the root of P. notoginseng, and by respectively 37.04%, 33.33%, 48.15%, 3.70% in the leaves, the safety concern of Cd and As should be strengthened. The enrichment coefficient of Cd in the root of P. notoginseng was the highest, and that of As and Cu was similar, while that of Pb was the smallest. The enrichment coefficient of Pb, As and Cu in leaves was small, which was similar to that of root, while the enrichment coefficient of Cd was different in different regions, and the largest was 8.3 times that of the smallest. Cluster analysis showed that the pollution degree of different heavy metals in different regions was significantly different. Pb, Cd, Cr and Cu of P. notoginseng from different regions accounted for 4.91%-10.00%, 3.83%- 10.50%, 7.34%-23.28% and 0.40%-0.61%, respectively, of the ingestion rates withADI (allowable daily intake). There are some risks of heavy metal pollution in Yunnan and this study can provide a theoretical basis for the standardized planting, safety evaluation and safety and quality control technology development of P. notoginseng.