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Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (9): 61-67.doi: 10.11924/j.issn.1000-6850.casb17100064

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Diversity Analysis of Culturable Bacteria in Intestine of Drosophila melanogaster and Drosophila suzukii

  

  • Received:2017-10-19 Revised:2019-03-15 Accepted:2018-01-24 Online:2019-03-26 Published:2019-03-26

Abstract: To understand the intestinal bacterial diversity in Drosophila suzukii and D. melanogaster, the bacteria in eggs, the intestine of larvae, pupae and adults of two Drosophila species were separated and purified, and identified with 16S rDNA genetic analysis. The results showed that, the number of bacteria was the lowest in eggs and the highest in larval intestine at different developmental stages of the two Drosophila species. And the number of bacteria in D. suzukii was more than that in D. melanogaster ignificantly. 31 species of bacteria were isolated from the two Drosophila species, the bacteria of Proteobacteria was the dominant population. The species of bacteria were 20 and 21 in D. suzukii and D. melanogaster, respectively. Therein, there were 13 species in both the two Drosophila species. The stable bacteria in intestine of D. suzukii were Citrobacter freundii, Klebsiella oxytoca, Chryseobacterium sp. and Lysinibacillus fusiformis. While, Acetobacter thailandicus, Paenibacillus taichungensis, Providencia rettgeri and Morganella morganii were stable bacteria in intestine of D. melanogaster. It is concluded that, the number and species of bacteria in D. suzukii and D. melanogaster are different at different development stages.