Abstract: To detect cultivation techniques of high and stable yield of rice and breeding new cultivars with resistance to high temperature under global warming condition, a field experiment is conducted to study the effects of high temperature during heading period on yield of 13 hybrid rice cultivars. The results showed that rice yield was significantly reduced by high temperature during heading period, the yield declined by 15.3% for each 1°C increase in this period. There were significant differences in response of yield of different hybrid rice cultivars to high temperature during heading period. The combinations of male parent Fengxinzhan had low seed setting rate (40.5%-59.3%), which had poorer tolerance to high temperature during this period. On the contrary, the combinations of male parent GR560 had greater tolerance to high temperature during heading period with high seed setting rate (64.9%-75.6%). To solve the problem of high temperature stress in rice heading period, more attention should be paid to breeding new cultivars with growth period suitable for the local cultivation environment, or adjusting cropping system and rice improving cultivation technique to ensure the crop to avoid high temperature during heading period.