Abstract: Based on the data of 64 disease indices and 38 disease field rates of 14 counties in Hedong of Gansu Province from 2001 to 2016, the most cold winter period was determined by the minimum mean distribution of 2、3、4～15 days sliding mean temperature from December to February. The overwinter meteorological condition statistical model of wheat stripe rust was established, using wheat stripe rust disease index and disease rate by stepwise regression method. Based on the results of the two methods, the suitable temperature index of wheat stripe rust overwinter in Hedong of Gansu Province was above - 9 ~ -10 ℃ of 7 days sliding mean temperature in the coldest period, and antecedent precipitation was more, the air relative humidity in the coldest period was higher, the suitable temperature was lower. The study have some guiding significance for publishing accurately the medium-short term trend forecast, actively developing early prevention and control the epidemic of disease.