Composition and Detection of High-molecular-weight Glutenin Subunit in Wheat: Research Progress

doi:10.11924/j.issn.1000-6850.casb17120116

Abstract

Abstract: The composition of high-molecular-weight glutenin subunit (HMW-GS) of wheat is closely related to the quality of bread. To select the most suitable detection method of HMW-GS to meet the test requirements, the authors introduced the composition of HMW-GS in wheat and the progress on detecting the composition of HMW-GS with three methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE), reverse phase high-performance liquid (RP-HPLC) and polymerase chain reaction (PCR). The authors discussed the advantages and disadvantages of the three methods, pointed out that SDS- PAGE and PCR methods were suitable for the HMW-GS detection in conventional breeding materials and cultivated wheat, and two-dimensional gel electrophoresis and RP-HPLC were suitable for the HMW-GS detection in the wheat with distant genetic material or the related species. At last, we proposed the application prospect of SDSPAGE and PCR in the molecular marker assisted breeding of wheat.

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