Abstract: The paper aims to study the effects of different hormones on the rooting of cutting of Amesiodendron chinense, and to increase the rooting rate by finding out suitable rooting methods. We explored the effects of 3 factors including hormone type, hormone concentration, and soaking time respectively on the rooting rate and healing rate by using the mature mother plant of A. chinense as the testing material. The results showed that: all the 3 factors (hormone type, hormone concentration, and soaking time) had significant impacts (P<0.05) on the cutting rooting rate and healing rate of A. chinense; the most significant factor was soaking time, not hormone type; hormone type and hormone concentration had impact on the healing rate of A. chinense and the impact of hormone type was greater than that of concentration; the rooting rate could reach to 62.22% when A. chinense were treated with IBA solution of 500 mg/L for 10 min.