Over-expression of OsCATb Enhanced E. coli Stress Tolerance to Heavy Metals

doi:10.11924/j.issn.1000-6850.casb18070019

Abstract

Abstract: The paper aims to study the mechanism of Oryza sativa Catalase b (OsCATb) in heavy metal stress. We introduced the exogenous reconstructed plasmid pGEX-6p-3-OsCATb into E. coli BL21. SDS-PAGE showed that there was a soluble protein band in 53 kDa in accordance with its predicted molecular weight. Under 1 mM Cd2+, Zn2+ or Cu2+ heavy metal stress, strain over-expressing GST-OsCATb exhibited better growth and increased tolerance than control (BL21) and E. coli over-expressing GST tag; meanwhile, the catalase activities in aim gene recombinant strain were also higher than the other two controls’. The results suggest that rice OsCATb appears to be an important antioxidant in cellular response, detoxification and tolerance processes under heavy metal stress.

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