Joint-toxicity Measurement of Pydiflumetofen and Tebuconazole to Fusarium pseudogramineaqum

doi:10.11924/j.issn.1000-6850.casb19010026

Abstract

Abstract:

To explore the joint-toxicity of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum, carry out the selection of efficient medicament, and provide a theoretical foundation for designing drug prophylaxes, through the conventional tissue isolation, Fusarium pseudogramineaqum strains were isolated from infected samples in Tai’an and surrounding areas, and the pathogenicity was measured. The indoor bioactivities of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum were detected by mycelium growth rate method. The results showed that EC₅₀ of pydiflumetofen and tebuconazole to Fusarium pseudogramineaqum was 0.107 mg/L and 0.906 mg/L, respectively, which indicated that the indoor bioactivity of pydiflumetofen was better than that of tebuconazole. Among the five ratios of pydiflumetofen and tebuconazole, the minimum EC₅₀ was found at ratio 1:1 (0.157 mg/L), and the maximum EC₅₀ was found at ratio 1:8 (0.303 mg/L). The SRs of these five ratios were 1.22, 1.54, 1.85, 1.98 and 1.63, among which the SRs of ratio 1:2, 1:4, 1:6 and 1:8 were greater than 1.5. It indicated that all these four ratios had synergistic effect on Fusarium pseudogramineaqum, and the effect of ratio 1:6 was the most obvious.

Key words: pydiflumetofen, tebuconazole, Fusarium pseudogramineaqum, joint-toxicity measurement, wheat basal rot

CLC Number:

S482.2

Dou Junxia, Geng Zhongyi, Zhao Jinglan, Sun Guobo. Joint-toxicity Measurement of Pydiflumetofen and Tebuconazole to Fusarium pseudogramineaqum[J]. Chinese Agricultural Science Bulletin, 2020, 36(13): 136-139.

Figures/Tables 2

References 27

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药剂	毒力回归方程	相关系数(r)	EC₅₀(ob)（95%置信区间）/(mg/L)	EC₅₀(th) /(mg/L)	增效系数SR	EC₉₀（95%置信区间）/(mg/L)
A氟唑菌酰羟胺	y=0.9698x+5.9425	0.989	0.107(0.078~0.145)	-	-	2.237(1.374~3.642)
B戊唑醇	y=1.1756x+5.0504	0.974	0.906(0.562~1.46)	-	-	11.152(5.858~21.226)
A:B=1:1	y=1.4824x+6.1915	0.988	0.157(0.112~0.22)	0.191	1.22	1.15(0.814~1.624)
A:B=1:2	y=1.3108x+6.0128	0.988	0.169(0.121~0.235)	0.259	1.54	1.603(1.112~2.311)
A:B=1:4	y=1.2556x+5.8892	0.985	0.196(0.135~0.285)	0.363	1.85	2.054(1.318~3.199)
A:B=1:6	y=1.4982x+5.9832	0.977	0.221(0.14~0.347)	0.438	1.98	1.582(0.944~2.651)
A:B=1:8	y=1.4197x+5.7356	0.960	0.303(0.167~0.549)	0.494	1.63	2.424(1.14~5.158)

药剂	毒力回归方程	相关系数(r)	EC₅₀(ob)（95%置信区间）/(mg/L)	EC₅₀(th) /(mg/L)	增效系数SR	EC₉₀（95%置信区间）/(mg/L)
A氟唑菌酰羟胺	y=0.9698x+5.9425	0.989	0.107(0.078~0.145)	-	-	2.237(1.374~3.642)
B戊唑醇	y=1.1756x+5.0504	0.974	0.906(0.562~1.46)	-	-	11.152(5.858~21.226)
A:B=1:1	y=1.4824x+6.1915	0.988	0.157(0.112~0.22)	0.191	1.22	1.15(0.814~1.624)
A:B=1:2	y=1.3108x+6.0128	0.988	0.169(0.121~0.235)	0.259	1.54	1.603(1.112~2.311)
A:B=1:4	y=1.2556x+5.8892	0.985	0.196(0.135~0.285)	0.363	1.85	2.054(1.318~3.199)
A:B=1:6	y=1.4982x+5.9832	0.977	0.221(0.14~0.347)	0.438	1.98	1.582(0.944~2.651)
A:B=1:8	y=1.4197x+5.7356	0.960	0.303(0.167~0.549)	0.494	1.63	2.424(1.14~5.158)

A氟唑菌酰羟胺			B戊唑醇			混配组合处理浓度/ (mg/L)	1:1(A:B)		1:2(A:B)		1:4(A:B)		1:6(A:B)		1:8(A:B)
处理浓度/ (mg/L)	病情指数/%	防治效果/%	处理浓度/ (mg/L)	病情指数/%	防治效果/%	混配组合处理浓度/ (mg/L)	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%
0.005	25.52	9.94	0.050	25.75	9.12	0.01	26.98	8.58	26.63	9.77	27.43	7.06	28.40	3.78	28.27	4.23
0.01	22.77	19.65	0.10	22.90	19.18	0.05	24.40	17.33	24.05	18.52	22.07	25.24	24.33	17.56	26.57	9.99
0.05	17.20	39.29	0.50	21.73	23.29	0.10	21.97	25.58	19.68	33.31	18.55	37.15	19.75	33.09	21.43	27.39
0.10	15.97	43.65	1.00	13.23	53.29	0.50	7.03	76.17	11.03	62.62	14.68	50.25	16.62	43.70	16.67	43.53
0.50	8.85	68.76	5.00	6.47	77.18	1.00	3.33	88.71	3.70	87.46	6.27	78.77	4.93	83.29	8.45	71.37
1.00	6.08	78.53	10.00	4.90	82.71	5.00	0.37	98.76	0.57	98.08	0.85	97.12	0.77	97.40	2.83	90.40
5.00	0.87	96.94	50.00	0.20	99.29	10.00	0.07	99.77	0.27	99.10	0.28	99.04	0.05	99.83	0.05	99.83
CK	28.33		CK	28.33		CK	29.52		29.52		29.52		29.52		29.52

A氟唑菌酰羟胺			B戊唑醇			混配组合处理浓度/ (mg/L)	1:1(A:B)		1:2(A:B)		1:4(A:B)		1:6(A:B)		1:8(A:B)
处理浓度/ (mg/L)	病情指数/%	防治效果/%	处理浓度/ (mg/L)	病情指数/%	防治效果/%	混配组合处理浓度/ (mg/L)	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%	病情指数/%	防治效果/%
0.005	25.52	9.94	0.050	25.75	9.12	0.01	26.98	8.58	26.63	9.77	27.43	7.06	28.40	3.78	28.27	4.23
0.01	22.77	19.65	0.10	22.90	19.18	0.05	24.40	17.33	24.05	18.52	22.07	25.24	24.33	17.56	26.57	9.99
0.05	17.20	39.29	0.50	21.73	23.29	0.10	21.97	25.58	19.68	33.31	18.55	37.15	19.75	33.09	21.43	27.39
0.10	15.97	43.65	1.00	13.23	53.29	0.50	7.03	76.17	11.03	62.62	14.68	50.25	16.62	43.70	16.67	43.53
0.50	8.85	68.76	5.00	6.47	77.18	1.00	3.33	88.71	3.70	87.46	6.27	78.77	4.93	83.29	8.45	71.37
1.00	6.08	78.53	10.00	4.90	82.71	5.00	0.37	98.76	0.57	98.08	0.85	97.12	0.77	97.40	2.83	90.40
5.00	0.87	96.94	50.00	0.20	99.29	10.00	0.07	99.77	0.27	99.10	0.28	99.04	0.05	99.83	0.05	99.83
CK	28.33		CK	28.33		CK	29.52		29.52		29.52		29.52		29.52