High-Salt Tolerance Porphyridium: Resources Screening and Its miR398-CSD Salt Stress Response Pattern

doi:10.11924/j.issn.1000-6850.casb2023-0263

Abstract

Abstract:

Porphyridium is a kind of characteristic microalgae with tremendous development potential. The purpose of this study is to explore and identify some high-salt tolerant Porphyridium strains, and to research their miR398-Cu, Zn-superoxide dismutase gene (CSD) salt stress response pattern, which will lay a foundation for the industrialized application of the special Porphyridium strains and exploring their salt tolerance mechanism. In this study, seven different Porphyridium strains were collected from home and abroad, and their morphological, physiological, phylogenetic evolution, and salt tolerance characteristics were analyzed, respectively. The high-salt tolerance strains among them were screened. Furthermore, the sequence of miR398 and its targeted gene CSD in Porphyridium were identified through the high-throughput sequencing and bioinformatics analyzing, respectively. Moreover, the inhibition type of miR398-CSD of the microalga was validated via molecular biology analysis. Lastly, based on the stem-loop qRT-PCR and qRT-PCR techniques, the expression patterns of miR398 and CSD in the high-salt tolerant Porphyridium strains under different salinity stresses were clarified. The results showed that, altogether, seven microalgae strains belonged to the Porphyridiumgenus, and were divided into three clusters via the phylogenetic analysis. Their cells morphology was consistent with the typical characteristics of Porphyridium. P1 and P4 were the high-salt tolerant strains. MiR398 inhibited its targeted gene CSD expression in the microalgae via slicing the gene targeted mRNA. The expression of miR398 in Porphyridium was down-regulated with the salinity increasing, while the CSD up-regulated. Our researches would lay a foundation for the development and utilization of highly-salt tolerant Porphyridium resources and molecular improvement of the novel algal strains based on the specific miRNAs.

Key words: Porphyridium, miR398, Cu/Zn-superoxide dismutase (CSD), salt-stress response

GAO Fan. High-Salt Tolerance Porphyridium: Resources Screening and Its miR398-CSD Salt Stress Response Pattern[J]. Chinese Agricultural Science Bulletin, 2024, 40(3): 103-111.

Figures/Tables 13

References 34

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编号	名称	大小	形状	颜色	蛋白核	载色体	来源
P1	P. marinum	H：12~13 μm；W：12~13 μm	圆形	紫色	有	有	中科院水生生物研究所
P2	P. aerugineum	H：10~11 μm；W：9~11 μm	圆形	紫红色	有	有	中科院水生生物研究所
P3	P. cruentum	H：13~15 μm；W：13~15 μm	圆形	紫红色	有	有	中科院水生生物研究所
P4	P. griseum	H：12~14 μm；W：12~13 μm	圆形	紫色	有	有	山西大学藻类实验室
P5	P. purpureum	H：12~14 μm；W：10~12 μm	椭圆形	紫色	有	有	山西大学藻类实验室
P6	P. sordidum	H：13~16 μm；W：13~15 μm	圆形	紫色	有	有	光语生物科技有限公司
P7	P. violaceum	H：14~17 μm；W：15~16 μm	圆形	紫红色	有	有	光语生物科技有限公司

编号	名称	大小	形状	颜色	蛋白核	载色体	来源
P1	P. marinum	H：12~13 μm；W：12~13 μm	圆形	紫色	有	有	中科院水生生物研究所
P2	P. aerugineum	H：10~11 μm；W：9~11 μm	圆形	紫红色	有	有	中科院水生生物研究所
P3	P. cruentum	H：13~15 μm；W：13~15 μm	圆形	紫红色	有	有	中科院水生生物研究所
P4	P. griseum	H：12~14 μm；W：12~13 μm	圆形	紫色	有	有	山西大学藻类实验室
P5	P. purpureum	H：12~14 μm；W：10~12 μm	椭圆形	紫色	有	有	山西大学藻类实验室
P6	P. sordidum	H：13~16 μm；W：13~15 μm	圆形	紫色	有	有	光语生物科技有限公司
P7	P. violaceum	H：14~17 μm；W：15~16 μm	圆形	紫红色	有	有	光语生物科技有限公司