With the medium containing different nutrition, Beauveria bassiana 13 was cultured to produce conidia powder in this experiment. The orthogonal experiment was carried out under different temperatures and the pH value, the different carbon and nitrogen source and the trace elements in the medium. Take the produced conidial content and sprouting rate as the major index, by screening, glucose was determined to be the best carbon source in which Beauveria bassiana 13 grew, the best nitrogen source was the protein peptone, the trace elements, copper and zinc, obviously promoted the production and sprout action of conidia powder, the most suitable pH value was 6~7, and the most suitable temperature was 20~25℃.
