Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (20): 68-71.
Special Issue: 生物技术; 棉花
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Abstract:
Abstract: In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli. Transformation efficiency of resistant plants about Jimian14 and Shann724 was 33% and 8.3% respectively. Effect of kanamycin concentration, the growth status of embryogenic calli and the genotype of the target material on the transformation efficicency had been assayed. The factors influencing agrobacterium-mediated cotton calli transformation were studied systematically in order to establish a highly efficient and stable genetic transformation system of cotton.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/
https://www.casb.org.cn/EN/Y2009/V25/I20/68