Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (20): 68-71.

Special Issue: 生物技术 棉花

Previous Articles     Next Articles

The Agrobacterium-Mediated PhyA Genetic Transformation for Embryogenic Calli of Cotton

  

  • Received:2009-05-22 Revised:2009-06-10 Online:2009-10-20 Published:2009-10-20

Abstract:

Abstract: In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli. Transformation efficiency of resistant plants about Jimian14 and Shann724 was 33% and 8.3% respectively. Effect of kanamycin concentration, the growth status of embryogenic calli and the genotype of the target material on the transformation efficicency had been assayed. The factors influencing agrobacterium-mediated cotton calli transformation were studied systematically in order to establish a highly efficient and stable genetic transformation system of cotton.