Abstract:

The total RNA was isolated from the PVY susceptible tobacco variety,the specific primers was designed according to the high homology and differential region of the published sequence of eIF4E gene,cloned the target gene fragment of eIF4E gene by RT-PCR. Then the promoter CaMV 35S driven,containing " Sense eIF4E gene target fragments-pdk intron-Antisence eIF4E gene target fragments",tobacco RNAi expression vector were constructed based on the intermediate vector pKANNIBAL and expression vector pART27. The RNAi plasmid is constructed to inhibit the expression of eIF4E gene,in order to understand the gene and lay the foundation for the breeding of tobacco viral resistance.