Abstract:

In order to increase the protein extraction rate from Job’s Tears seed, determine the pH, solid-liquid ratio, treatment temperature, time on the extraction rate of protein effects, further barley protein SDS-PAGE electrophoresis. Based on single-factor test, the optimum extraction conditions were confirmed by response surface method, and made SDS-PAGE analysis of Job’s Tears seed electrophoresis. The results showed that, the optimum extraction conditions were confirmed by response surface method as follows: solid to liquid ratio 1:11.8, pH 10.47, treatment temperature 35℃, and time 4.93 h. Under these conditions, the extraction rate was 43.94%. The SDS-PAGE analysis showed that, the proteins from Job’s Tears seed contained approximately 2 subunits, larger barley protein subunit molecular weight was 143 kD, little barley protein subunit molecular weight was 85 kD. Through the response surface alkali extraction from Job’s Tears seed, extraction rate increased significantly, the SDS-PAGE method was adopted to analyze, to construct the protein fingerprint and provide a theoretical basis.