Abstract: Aims at the high application amount of fertilizer, the unreasonable fertilization structure, the low fertilizer use efficiency, the backward mode of fertilization and the gradually increasing application amount of fertilizer in Yunnan Province, the authors collected 410095 questionnaires of“Investigation on Fertilization of Soil Testing and Formulated Fertilization”, analyzed fertilization status of 410095 crop seasons of 351194 plots in 16 cities of Yunnan, and summarized the harm of excessive application of chemical fertilizer. Fertilization methods and technologies of appropriate kind, time and amount were suggested, combined with scientific field management and“fertilizer + ’to achieve the goal of zero growth of fertilizer. The results could provide guidance for agricultural production in order to ensure food security in Yunnan.