Heterologous Expression of BvHSP18.2 Gene in Sugar Beet Enhancing Tolerance to Cadmium of Arabidopsis thaliana

doi:10.11924/j.issn.1000-6850.casb2024-0265

Abstract

Abstract:

In order to further analyze and verify the function of the sugar beet BvHSP18.2 gene (LOC104903994) regulated by cadmium stress, we cloned the sugar beet BvHSP18.2 gene by RT-PCR in this study, and measured the phenotypic and physiological phenotypes and physiological changes of Arabidopsis thaliana plants heterologously expressing the target gene under different concentrations of cadmium stress by plant expression vector construction as well as Arabidopsis thaliana genetic transformation. The phenotypic and physiological changes of Arabidopsis thaliana plants heterologously expressing the target gene under different concentrations of Cd were measured. The results showed that with the increase of cadmium stress concentration, Arabidopsis thaliana plants heterologously expressing the BvHSP18.2 gene had an advantage in both root length and fresh weight. The largest difference was 1.1 cm in average root length under 50 µmol/L cadmium stress, and the largest difference was 0.015 g in average fresh weight under 600 µmol/L cadmium stress, and the overexpression of the BvHSP18.2 gene could effectively increase the S-phase of Arabidopsis thaliana plants under different concentrations of cadmium stress. The overexpression of BvHSP18.2 gene enhanced the tolerance of Arabidopsis thaliana under cadmium stress, and it was hypothesized that this gene played an important role in the adaptation of plants to cadmium stress.

Key words: sugar beet, Arabidopsis thaliana, BvHSP18.2 gene, heterologous expression, cadmium stress, functional analysis, gene cloning, sod activity, pod activity, tolerance, genetic transformation

ZHONG Weiting, ZHANG Qiong, XING Wang, LIU Dali. Heterologous Expression of BvHSP18.2 Gene in Sugar Beet Enhancing Tolerance to Cadmium of Arabidopsis thaliana [J]. Chinese Agricultural Science Bulletin, 2024, 40(29): 14-20.

Figures/Tables 6

References 35

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