Effect of Pollination Varieties on Fecundity and Fruit Quality of Blackberries ‘Freedom’

doi:10.11924/j.issn.1000-6850.casb2025-0750

Abstract

Abstract:

In order to investigate the effects of pollen xenia on the fruiting and fruit quality of ‘Freedom’ blackberry, this study used ‘Freedom’ as the recipient and conducted artificial pollination with four male parent varieties, including ‘Ark-45’, ‘Erika’, Rubus eustephanos and Rubus peltatus. Comprehensive analysis and evaluation were performed on fruiting, fruit quality, and seed-related indicators. The results indicated that ‘Freedom’ exhibited pollen xenia. The fruit setting rate (54.49 % - 86.01 %), single fruit weight (0.87 - 11.28 g) and soluble solids (8.59 % - 16.53 %) of different male parents were significantly different. Principal component analysis extracted three principal components, with a cumulative contribution rate of up to 95.55%, reflecting indicators such as fruit size, flavor, and seed size. The comprehensive evaluation showed that the pollination effect of Rubus peltatus (comprehensive score 1.94) and Rubus eustephanos (1.88) was the best, followed by 'Ark45' (0.41) and 'Erika' (0.09). In summary, Rubus peltatus and Rubus eustephanos are suitable pollinating varieties of ‘Freedom’ blackberry, and the xenia effect of pollen can effectively improve its comprehensive quality. In the future, ploidy analysis and physiological mechanism research can be combined to clarify the regulation path of pollen xenia, so as to provide more accurate support for pollination configuration and quality improvement of blackberry.

Key words: blackberry, artificial pollination, pollen xenia, fecundity, fruit quality, comprehensive evaluation, pollination varieties

GUAN Jiyuan, CHEN Zhe, ZHANG Qunying. Effect of Pollination Varieties on Fecundity and Fruit Quality of Blackberries ‘Freedom’[J]. Chinese Agricultural Science Bulletin, 2026, 42(5): 77-82.

Figures/Tables 6

References 27

[1]	吴文龙, 李维林. 黑莓引种栽培与利用[M]. 南京: 江苏科学技术出版社,2010:1-2.
[2]	RAMESHWAR V, TUSHAR G, RAKESH P, et al. Rubus fruticosus (blackberry) use as an herbal medicine[J]. Pharmacognosy reviews, 2014, 8(16):101-104. doi: 10.4103/0973-7847.134239 URL
[3]	李维林, 吴文龙, 张春红, 等. 世界黑莓产业发展和研究现状及前景[J]. 植物资源与环境学报, 2012, 21(3):105-115.
[4]	洪俊彦, 黄仁, 黄春颖, 等. 植物花粉直感的研究进展及展望[J]. 植物生理学报, 2020, 56(2):151-162.
[5]	王宝侠, 韩永增, 包敖民, 等. 授粉品种对‘塞外红’苹果果实品质的影响[J]. 经济林研究, 2023, 41(4):22-30.
[6]	曾莎芮, 马瑞丰, 刘蕊, 等. 不同品种授粉树对梅州金柚果实品质的影响[J]. 中国果树, 2025(8):68-77.
[7]	何溢, 徐宸宇, 朱婉婉, 等. 贡水白柚不同授粉组合亲和性鉴定及品质改良[J]. 中国南方果树, 2024, 53(S1):12-20.
[8]	伏芳, 武雅娟, 姜燕, 等. ‘京白梨’果形及果重差异影响因素分析[J]. 中国农学通报, 2024, 40(7):39-43. doi: 10.11924/j.issn.1000-6850.casb2023-0160
[9]	李皓, 李湘钰, 吴翠云, 等. 花粉直感对‘新梨7号’果实品质的影响[J]. 西北农林科技大学学报(自然科学版), 2026(1):45-56.
[10]	鲁晓峰, 孙海龙, 刘硕, 等. 花粉直感对李果实香气成分的影响[J]. 经济林研究, 2024, 42(4):87-98.
[11]	李学铃, 刘英, 吴文龙, 等. 不同倍性蓝莓杂交组合的结实特性及果实感官品质研究[J]. 南京林业大学学报(自然科学版), 2025, 49(3):41-51. doi: 10.12302/j.issn.1000-2006.202404027
[12]	杨芩, 邓洁, 张婷渟, 等. 花粉直感效应对蓝莓果实花青素合成及其关键酶活性的影响[J]. 北方园艺, 2024(16):15-21.
[13]	侯延杰, 李鸿莉, 邱宏业, 等. 花粉直感对“仙进奉”荔枝效应的研究[J]. 中国南方果树, 2022, 51(4):53-57.
[14]	徐祥增, 邓乐晔, 张小娇, 等. 柚和葡萄柚花粉直感对东试早柚果实生长及品质的影响[J]. 果树学报, 2024, 41(4):665-678.
[15]	张武杰, 高洪娜, 汤佳翰, 等. 花粉直感对黑穗醋栗果实品质的影响[J]. 北方园艺, 2025(16):69-78.
[16]	FINN C, MENG R. Determining ploidy level and nuclear DNA content in Rubus by flow cytometry[J]. Journal of the American society for horticultural science, 2002, 127(5):767-775. doi: 10.21273/JASHS.127.5.767 URL
[17]	刘英, 李学铃, 闾连飞, 等. 异倍体黑莓授粉结实性及果实性状分析[J]. 植物资源与环境学报, 2025, 34(3):53-65.
[18]	郑东生, 杨荣, 聂建文, 等. 不同授粉品种对‘苹果梨’果实的花粉直感效应[J]. 北方果树, 2025(1):10-14.
[19]	叶田诚, 潘松, 许贺然, 等. 花粉直感效应对长江1号软枣猕猴桃果实品质的影响[J]. 中国果树, 2024(6):47-56.
[20]	李爱华, 向珊珊, 赵剑平, 等. 香榧花粉直感现象研究[J]. 农学学报, 2022, 12(4):54-56. doi: 10.11923/j.issn.2095-4050.cjas2020-0094
[21]	毛桑隐, 路志浩, 张祥, 等. 花粉直感对马家柚果实品质的影响[J]. 果树学报, 2023, 40(11):2391-2402.
[22]	张群英, 文光琴, 李永霞, 等. 优良树莓品种及当地野生树莓的引种比较研究[J]. 种子, 2015, 34(1):100-103.
[23]	王小安, 陈文光, 郭云伟, 等. 4个保加利亚树莓和黑莓品种在福建引种表现及其栽培技术[J]. 中国南方果树, 2019, 48(2):121-124.
[24]	毕光林, 陈林杨, 杨永智, 等. 51份柚类种质果实品质分析与综合评价[J]. 热带作物学报, 2025, 46(11):2633-2644. doi: 10.3969/j.issn.1000-2561.2025.11.009
[25]	宋光永, 郭雅文, 刘春燕, 等. 和田设施中十八个鲜食葡萄品种果实品质比较[J]. 北方园艺, 2024(21):37-43.
[26]	徐宸宇, 唐启正, 刘慧宇, 等. 基于主成分分析综合评价6个杂交授粉组合的马家柚果实品质[J]. 果树学报, 2024, 41(2):282-293.
[27]	王峰伟, 贾芸, 张莹, 等. 不同雄株花粉对‘秦紫1号’黑蕊猕猴桃果实品质的影响[J]. 陕西林业科技, 2023, 51(4):5-10.

母本	父本	坐果率/%	成熟期(月-日)
‘自由’	‘自由’(CK)	100.00A	05-20
	‘Ark-45’	86.01B	05-23
	‘伊瑞卡’	83.83C	05-28
	大红藨	63.30D	05-30
	盾叶莓	54.49E	05-25

母本	父本	坐果率/%	成熟期(月-日)
‘自由’	‘自由’(CK)	100.00A	05-20
	‘Ark-45’	86.01B	05-23
	‘伊瑞卡’	83.83C	05-28
	大红藨	63.30D	05-30
	盾叶莓	54.49E	05-25

母本	父本	单果重/g	果实纵径/mm	果实横径/mm	果形指数	可溶性固形物/%	可滴定酸/%	固酸比
‘自由’	‘自由’(CK)	12.40±0.25A	40.27±3.17A	27.53±0.91A	1.47±0.17A	10.13±0.12C	0.33±0.02C	31.07±1.75A
	‘Ark-45’	11.28±1.43A	36.38±2.27B	26.28±0.48AB	1.35±0.03A	8.79±0.76D	0.63±0.30AB	20.31±0.63D
	‘伊瑞卡’	8.16±0.97B	30.26±1.41C	24.25±0.25B	1.31±0.09A	8.59±0.62CD	0.32±0.03C	28.32±1.01B
	大红藨	5.17±0.12C	27.07±0.89C	19.42±1.54C	1.40±0.11A	13.77±0.55B	0.41±0.02BC	33.53±1.57A
	盾叶莓	0.87±0.05D	9.28±0.53D	13.66±0.47D	0.68±0.06B	16.53±0.46A	0.70±0.03A	23.52±0.45C

母本	父本	单果重/g	果实纵径/mm	果实横径/mm	果形指数	可溶性固形物/%	可滴定酸/%	固酸比
‘自由’	‘自由’(CK)	12.40±0.25A	40.27±3.17A	27.53±0.91A	1.47±0.17A	10.13±0.12C	0.33±0.02C	31.07±1.75A
	‘Ark-45’	11.28±1.43A	36.38±2.27B	26.28±0.48AB	1.35±0.03A	8.79±0.76D	0.63±0.30AB	20.31±0.63D
	‘伊瑞卡’	8.16±0.97B	30.26±1.41C	24.25±0.25B	1.31±0.09A	8.59±0.62CD	0.32±0.03C	28.32±1.01B
	大红藨	5.17±0.12C	27.07±0.89C	19.42±1.54C	1.40±0.11A	13.77±0.55B	0.41±0.02BC	33.53±1.57A
	盾叶莓	0.87±0.05D	9.28±0.53D	13.66±0.47D	0.68±0.06B	16.53±0.46A	0.70±0.03A	23.52±0.45C

母本	父母	种子厚度/mm	种子长/mm	种子宽/mm	种形指数	种子数/粒	百粒重/g
‘自由’	‘自由’(CK)	1.07±0.06A	3.39±0.10A	1.91±0.05A	1.77±0.08AB	141.33±4.93A	0.36±0.01A
	‘Ark-45’	0.92±0.01B	2.86±0.02B	1.68±0.04B	1.70±0.05AB	134.00±10.51A	0.31±0.01B
	‘伊瑞卡’	0.90±0.04B	2.78±0.05B	1.68±0.06B	1.66±0.07B	78.33±5.13B	0.26±0.01C
	大红藨	1.15±0.08A	2.61±0.11C	1.70±0.07B	1.54±0.06C	21.33±1.53C	0.24±0.00C
	盾叶莓	0.96±0.05B	2.38±0.06D	1.32±0.03C	1.81±0.06A	11.33±1.53C	0.10±0.01D