Abstract:

A total of eleven reference genes were systematically compared in different tissues of Rehmannia glutinosa using RT-qPCR, including seven traditional reference genes (ACT11, 18S, TUB5, GAPDH, EF-1?, UBQ10 and UBQ5) and four novel candidates reference gene (HSP90, PP2A, RPⅡ and TIP41) in this study. The mRNA levels of these genes were analyzed among the organs of flowers, roots, stems and leaves representing the vegetative growth phase and the generative growth phase. The stabilities of the eleven candidate reference genes were ranked by the Ct value comparison and three softwares (GeNorm, NormFiner and BestKeeper). The results showed that the expression of TIP41 and UBQ10 was stable in different tissues of sepals, petals, stamens, pistils, and ovary from flower. For the case of the roots, stems and leaves sampled in vegetative growth phase and generative growth phase, UBQ5 andTIP41 were expressed stably. As a result, the two groups of candidate genes were selected as reference genes for Rehmannia glutinosa.