Abstract:

The random amplified polymorphic DNA (RAPD) markers technique was used to analyze the genetic diversity among P. salicina×P. Atropurupurea and Prunus salicina cv. ‘angenuo’ and 10 hybrids. 15 primers were screened from 100 random primers, and they were amplified in all hybrids. The polymorphic bands, the percentage of polymorphic loci, Shannon information index (I), Nei's genetic diversity index (H), genetic distance and genetic identity were analyzed by using PopGene3.2 software. The dendrogram based on genetic distance was constructed by using DPSv2.0 software, in accordance with the un-weighted pai-group method using an arithmetic average (UPGMA). The results showed that 15 primers amplified 89 polymorphic bands, the total percentage of polymorphic loci was 93.54%, the average Shannon information index was 0.4298, and the average Nei's genetic diversity index was 0.3574, which indicated more genetic diversity of offspring. The dendrogram showed the genetic variation of hybrids was rich. The No.1 had the closest relationship with the parent plant, which indicated it could inherit the red leaves traits of the female parent likely.