Abstract:

As an efficient tool to measure the nuclear DNA content and ploidy level, flow cytometry has been playing very important role in many fields of botany research. Through plenty of flow cytometry experiments, a set of universal experimental procedure for detecting the nuclear DNA content and ploidy level was summarized in the laboratory of molecular ecology, Lanzhou University. At the same time, we had elaborated key points at every experimental stage and solutions to the problems caused by too much fragments which could result in the failure of an experiment, which might provide guidance for the future experiments, and promote applications of flow cytometry in plant research.