Abstract:

Genetic analysis of released and artificial cultured urchins, Strongylocentrotus intermedius, were conducted with microsatellite technology in this research. [Methods]Seven pairs of primers were amplified in all individuals of each urchin population. Results showed that 1 to 5 alleles had been amplified in each of detected loci. In total, 22 alleles were detected in cultured population, while 17 of them in released population. Genetic indicators calculated by Popgene32 software showed that there were no significant differences between these two populations in the Nei’s index, the Shannon's index and the proportion of Polymorphic loci, which indicates that there are no significant genetic differentiations between released and artificial cultured population. There are six alleles were detected in cultured population but not in released ones, while 1 allele were found in released population but not in cultured ones. Both results indicates that there are still some allele variations existed between cultured and released urchin populations. Results from this research shows that the releasing program is failed to improve germplasm resources of S. intermedius. This research have found the genetic structure of cultured and released urchin populations, which can be a basis of theories and strategies of artificial culture and releasing for enhancement in S. intermedius.