Abstract:

This paper described an adventive bud information from callus by new sprout for in vitro propagation of adult Pinus massoniana Lamb. Emphasizing on the callus maintenance, proliferation and differentiation, experiments were conducted to discuss osmotic pressure by inositol, hormone in the medium, as well as culture conditions. Results as followed: (1) adding inositol 3.0 g/L in the modified GD medium could effectively solve the browning problem and maintaining the callus proliferation; (2) To switch the callus culture condition from dark into light environment, adding KT 1.0-2.0 mg/L and NAA 0.5 mg/L in the DCR medium was viable; (3) DCR medium with KT 1.0 mg/L and thidiazuron (TDZ) 0.5 mg/L could promote the callus differentiate into adventive bud and 4 buds achieved. This protocal provided foundation for establishing a system of micropropagation with the superior genotype of Pinus massoniana L.