Abstract:

In order to study the application of GmMYB12B2 gene in transgenic plants involving flavonoids metabolism, the plant expression vector PPZP-GmMYB12B2 was constructed, in which the GmMYB12B2gene was driven by the constitutive promoter CaMV35S. The constructed expression vector was transformed into EHA105 strain by freeze -thaw method. Leaf segments of tobacco were inoculated with EHA105 containing plasmid PPZP-GmMYB12B2. The transgenic lines were selected by PCR detection, in nine random transgenic tobaccos, six of them were positive plants. The result showed that GmMYB12B2 gene had been integrated into the genome of tobacco.