Abstract:

Conventional methods for RNA extraction are not feasible to mature seeds of gramineous crops for the enrichment of polysaccharide, protein and lipid. In this paper an improved method for isolating total RNA from the seeds of wheat, rice and maize is introduced. Comparing with the Conventional methods for RNA extraction, the improved method was of high purity (with the ratio of OD260/OD280 1.6~1.8) and integrity and the results of RT-PCR and Northern blot showed that the RNA extracted by this improved method can meet the experimental requirements for high quality RNA, such as microarray, small RNA library construction for subsequent Illumina sequencing.