Abstract:

To stop the strains confusion for the timely, the DNA fingerprint analysis was used in the study of 11 strains of Pleurotus ferula which popular culture in the country. We mainly used RAPD, ISSR, SRAP polymorphism analysis method, and the 8 primers of RAPD, ISSR, SRAP which We screened produced 116 band-types of DNA fragments, and cluster analysis lastly. The results showed that all strains could be clustered into four groups, ClassⅠ: Pl. f 0007; ClassⅡ: Pl. f 0002; ClassⅢ: Pl. f 0003; ClassⅣ: The remaining 8 strains. The study not only provides the evidence for the foundation of the core collections of Pleurotus ferula and the scientific preservation of resources, but also lays a foundation for regulating the production of Pleurotus ferula.