Abstract:

For invent the new germplasm line by mutator gene, we had been injected the aqua of pingyangmycin in each concentration between 0-50 μg/mL into the growing point in stem tip of the wheat ‘Lankaoaizao8’. There were many descendants had been mutated in plant height, waxiness, awn, ear type, chlorophylle, maturation. We had owned many unaltered germplasm line by subsequent continuous selection in the variational descendants. Detect the DNA of ‘Lankaoaizao8’、pyms-6、 pyms-2 using SSR markers. There were many differential fragments which had been found by the Prime xgwm148, xgwm645, xgwm6, xgwm107, xgwm213, xgwm219, xgwm260, xgwm400, xgwm295. In molecular level，It had been attested that Injected the aqua of pingyangmycin into the growing point in stem tip of the wheat at growth phase is a feasible program to Induced mutator gene of the wheat.