Abstract:

The research advances in the assay methods of the lipoxygenase activity in higher plants were systematically summarized in this review. The normal assay methods involve two categories. One is based on the rate determination of the hydroperoxide produced in the lipoxygenase-catalyzed reactions, including carotene bleaching, Fe(CNS)3 coloration, standard spectrophotometric, 2-thiobarbituric acid coloration, potassium iodide-starch and xylenol orange methods. The other is based on the determination of the oxygen-absorbing rate of the substrates in the lipoxygenase-catalyzed reactions, including manometric, oxygen electrode and methylene blue staining methods. Other assay method includes enzyme linked immunosorbent assay, superweak luminescence emission spectrum assay and radioisotopic labelling method. Among all of the above methods, the standard spectrophotometric and the oxygen electrode methods are both regarded as the standard ones. This paper could provide a reference for the efficient and accurate determination of the lipoxygenase activity in the specific higher plants.