Abstract:

Ethylene signaling plays important roles in multiple processes of plant growth and development, ACO gene is the key factor regulating biosynthesis of ethylene, however, functions of ACO gene and ethylene in abiotic stress responses remain largely unknown. Here, we cloned a cotton GhACO2 gene from cotton fiber cDNA and constructed a plant overexpression vector p35S::GhACO2. Genetic transformation of Arabidopsis was performed by floral dip method, successive selection were completed with Kanamycin and further PCR and GUS histochemical detection of transgenic arabidopsis plants. The results of selection showed that GUS enzyme of transgenic arabidopsis plants was expressed greatly in leaf and little in stem and root, GhACO2 gene was integrated in the Arabidopsis genome and transgenic positive plants were obtained successfully. The T2 generation homozygous transgenic plants were obtained by selfing, and were used to undergo salt-stress and drought-stress treatment by NaCl and PEG6000. The results indicated that, with the comparison of Col wide type plants, stress tolerances of transgenic plants were enhanced significantly. All these results establish the basis of researching biological functions of GhACO2 further and transgenic breeding by Genetic engineering technology.