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Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (8): 108-113.

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Primary Studies on Breeding Techniques by 2n Gametes in colored Zantedeschia hybrid

  

  • Received:2011-01-26 Revised:2011-02-17 Online:2011-04-20 Published:2011-04-20

Abstract:

The cultivars of colored Zantedeschia hybrid: Black magic and Flamingo are used as experimental material. The young robust buds were selected to be treated with 0.15% colchicine to induce 2n gametes. The treated young flower buds between the two cultivars were crossed when pistil and stamena matured. The results showed that: colchicine had some poison impact on the development of the pistil and androecium, especially on that of pistil; 2n gametes can overcome sterility of hybrids to a certain extent. Under the same conditions, the seed germination of hybrids derived from treated gametes was sped up greatly (about 50d in advance) compared with that of control seed; the leaves were larger, thinner, and light color, and some plants performed malformation. The chromosome identification showed: triploids were achieved from the crossing of treated gametes with normal gametes and the chromosome number was 32 (2n=3x=48).