Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (16): 61-64.

Special Issue: 资源与环境

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Analysis of Lycium chinense Variety Revealed by RAMP-PCR Method

  

  • Received:2011-01-26 Revised:2011-03-02 Online:2011-07-05 Published:2011-07-05

Abstract:

To develop valid methods of DNA extraction for Chinese wolfberry for RAMP analysis and research. The result showed that: ten primers were applied to amplification. A total of 87 DNA bands were detected. 53 bands among which were polymorphic. The rate of polymorphic bands was 33.3%-81.9%. 10 Chinese wolfberry cultivars genetic distances were from 0.1111 to 0.8333. In the study, the RAMP markers can be used for the studies of fingerprint of Chinese wolfberry sensitively.