Abstract:

In order to obtain an ideal total RNA extraction method of Ginkgo biloba L. providing foundation for molecular biology research, total RNA from leaves at different growth stages were extracted with three methods, including CTAB method, ethylene glycol monobutyl ether method and a modified Trizol method, and its quality and quantity were compared. The results indicated that the modified Trizol method was better than the other methods since the value of OD260/OD280 and OD260/OD230 were 1.8-2.0 and 1.9-2.1, the purities were higher, the bands of 28S and 18S rRNA on gel electrophoresis were very clear and did not degradated. Comparably, CTAB method was not very good as the first method because RNA was easy degraded and dispersed although the value is higher, and ethylene glycol monobutyl ether method was not fit the requirement of RNA extraction.