Abstract:

In order to solve the long cycle of conventional breeding of cotton and the difficult regeneration, the low conversion rate and high rate of embryo variability in Agrobacterium-mediated transformation of hypocotyl of cotton. Agrobacterium-mediated transformation using cotton tip as explants, by improving the genetic transformation of the tip, try to shorten the conversion period and get into seedlings.The fungal elicitor gene pemG1 was cloned from Magnaporthe grisea and its plant expression vector pCAMBIA2300-pemG1 was constructed. The apical meristems of cotton cultivar ‘Coker312’ and ‘CCRI24’ were used as explants for agrobacterium-mediated transformation. The optimum co-culture temperature, incubation time and the concentration of kanamycin were determined. The 0.1 mg/L of BAP and NAA was selected as the best concentration for bud induction. After subculture for two months, the stem was transferred to the medium containing GA3 for proliferation and elongation, and then to the medium containing 0.1 mg/L of IBA for rooting. Finally, the resistant plants were transplanted in the greenhouse and 4 transgenic plants were acquired by preliminary molecular verification.