Abstract:

Potato Shepody is economically important potato cultivar for french fries in the world. However, this cultivar exhibits very susceptible to severe environmental stress. The aim of this study was to develop an efficient plant transformation system for potato cultivar Shepody and to obtain transgenic potatoes. Leaf disks, stem segments and minitubers from potato cultivar Shepody were used as receptor materials to compare transformation efficiency with different Agrobacterium tumefaciens content, duration of infection and the proportion of hormones. The results showed that leaf disks was most suitable for the induction of callus and shoots; Leaf disks explants infected by A.tumefaciens strain Agl-0 (OD600=0.5, 10 min) and selected on MS20 containing 1.0 mg/L NAA and 1.0 mg/L zeatin-trans(ZT-t), yielded the highest transformation efficiency with 95% of callus induction and 20% of differentiation. The gene resistant to potato late blight (R1) was introduced into potato through the above transformation system. PCR analysis to the kanamycin-resistant transformants manifested that the R1 gene was integrated into the potato genome.