Abstract:

The aim was to screen the best extraction technology of complex prescription of traditional Chinese medicine Modified Pulsatilla’s effective parts. The extract yield of effective parts, minimal inhibitory concentration (MIC) of extracts to Escherichia coil and minimum bactericidal concentration (MBC) of extracts to Escherichia coil were used as three indicators comprehensive evaluation. The appropriate concentration of solvent was screened by single factor test. The best extraction technology was screened by orthogonal design with four factors, which were solid-liquid ratio (A), extraction time(B), each time of extraction (C) and concentration of ethanol (D). The best method of extraction was reflux extraction. The best combination was A3B3C1D2, which means solid-liquid ratio was 1:10, extraction time was 3, each time of extraction half an hour at a time and concentration of ethanol was 60%. The rate of complex prescription extraction is about 24% with the optimum technology, both MIC and MBC to Escherichia coil were 15.625 mg(crude drug)/mL.