Abstract:

Ratoon stunting (RSD) is a major disease of sugarcane affecting sugarcane yield, sucrose and ratoon sugarcane years, and one of the important reasons of sexual degradation, causing seriously economic loss. In this paper, in order to investigate the occurrence of ratoon stunting disease at all levels and to provide scientific guidance for the nursery plantlets production, an established ratoon stunting virus PCR detection technology was used to monitor ratoon stunting disease-free seed production of plantlets or nursery seedlings, The results showed that RSD were not detected in the nursery in the new kinds of primary and secondary health plantlets, like ‘YT00-236’ and ‘ROC22’, while RSD detection rates in nursery perennial species of a health were 20% and 5%, respectively. In addition, RSD detection rates at all levels of health of the total species of nursery were 6.7% and 2%, which were reduced by 86.6% and 68%, respectively, compared with the common stem seedlings. Thus, our results indicated that the RSD bacteria had been effectively removed in the established nursery healthy sugarcane plantlets.