Abstract:

In order to achieve the factory peony nursery to provide theoretical basis, establish the tissue culture system of Peony ‘ling hua zhan lu’. Use ‘ling hua zhan lu’ buds (axillary buds, sprout buds, flower buds) as explants to MS, improved MS, WPM, WPM improved as the basic medium with different concentrations of 6-BA, NAA and other plant growth regulators, were screened by comparing test ‘ling hua zhan lu’ the best medium for improved differentiation WPM +6-BA 1.5 mg/L + NAA 0.5 mg/L + GA3 0.1 mg/L + sugar 25 g/L + agar 6 g/L + AgNO3 10 mg/L. Multiplication coefficient 3.7. Rooting in different concentrations of carbon, rooting medium was 1/2 modified WPM + IBA 10 mg/L + sugar 35g/L + AgNO3 10 mg/L+ activated carbon (0.1%) when the rooting rate was 47.9%.