Abstract:

The uncertainty of panax notoginseng saponins was analyzed by spectrophotometric method, in order to find the factors that affect the uncertainty and provide basis for improving determination methods and evaluating test reports. The panax notoginseng saponins was determined by spectrophotometry and the uncertainty was assessed according to JJF 1059—1999 Uncertainty Assessment and Expression. A mathematical model was established and the end-result of each component was found out. According to the experimental determination, estimating of class A is caused by repeatability influence. Relative uncertainty of 0.00296 has been obtained through statistical analysis of observation column by many parallel determinations in this article. Quality components, volume components and spectrophotometer absorbance components were included in estimating of class B，Relative uncertainty of 0.00321. The uncertainty has been calculated through standard curve regression equation and was caused by standard curve fitting. The calculation of relative uncertainty was obtained for . According to the analysis, this assessment uncertainty of 0.22% was described. This method had certain accuracy and can meet the requirement determination. In this uncertainty evaluation, uncertainty components, caused by weighing scales and method of measurement repetitive, had a significant contribution to synthetic uncertainty.