Abstract:

This article described a simple recovery method of DNA from polyacrylamide gels, direct recovery method. The aim was to prove the high efficiency of direct recovery method for DNA recovery from polyacrylamide gels, compared with traditional boiling method. With cassava cDNA as experimental material, this method and boiling method were compared from three aspects: experimental method, extract and amplified rates, pollution rates. The results showed that direct recovery method was more simple and fast than boiling method, pollution rates of boiling method was 59.09%, and the direct recovery method was 43.08%, the average amplification products by boiling method about 40 ng/μL, the direct recovery method was 60 ng/μL. The results indicated that the direct recovery method was easier, faster, and efficient. It had lower pollution rates.