Abstract:

In order to explore an easily industrialized and lower costs method for flavonoids extraction from medical plants cells, technique was studied to provide theoretical foundations and practical directions for industrial production. Total flavonoids which were from Ginkgo biloba callus were extracted and determined. ethnol extraction, ultrasonic technology and water immersion extraction were used in this experiment. The content of total flavonoids was determined by spectrophotometric methods. The optimal conditions in ethnol extraction can be gotten as follows: ratio of material to solvent in 80% ethanol = 1∶7 (m∶v), temperature 60℃, extracting time thrice and the extracting ratio 3.67%. The optimal conditions in ultrasonic technology were that ratio of material to solvent in 60% ethanol = 1∶34 (m∶v)，the time 65 min and the extracting ratio 4.37%. The optimal conditions in water immersion extraction can be gotten as follows: ratio of material to solvent in water= 1∶30 (m∶v)，temperature 90℃, the time 3.5 h and the extracting ratio 2.95%. The order of flavlnoids extracting ratio in three optimized methods was ultrasonic technology＞ethnol extraction＞water immersion extraction.