Abstract:

Three sets of specific primers (SCMV-F/R, SrMV-F/R, SCYLV-F/R) for each virus based on the CP gene sequence of sugarcane mosaic virus (SCMV), sorghum mosaic virus (SrMV) and sugarcane yellow leaf virus (SCYLV). Total RNA was extracted from sugarcane leaf with mosaic and yellow leaf symptom. The expected 850 bp and 630 bp fragment was amplified by RT-PCR with SrMV-F/R and SCYLV-F/R, respectively. Sequence analysis indicated 850 bp segment shared identities of 95% homologus with SrMV isolates from Xiangshan, Linping and Yuhang in Zhejiang Province (GenBank accession numbers AJ310194, AJ310195 and AJ310198). 630 bp segment shared identities of 99% homologus with SCYLV isolates from Guangdong, Guangxi and Fujian (GenBank accession numbers GU190165, GU190162, GU190159). Sequence analysis confirmed the sugarcane was co-infected by SrMV and SCYLV. A double RT-PCR was applied to detecting SrMV and SCYLV base on single RT-PCR. Sensibility test showed it could simultaneously detect out SrMV and SCYLV from 10-6 g sugarcane leafs infected by virus. It was proved simple, rapid and sensitive for detection of SrMV and SCYLV.